Full Cast — Pee-wee's Playhouse (1986–1990)

Paul Reubens

Pee-wee Herman

Lynne Marie Stewart

Miss Yvonne

Laurence Fishburne

Cowboy Curtis

S. Epatha Merkerson

Reba

John Paragon

Jambi

Alison Mork

Chairry (voice) / Magic Screen (voice)

Suzanne Kent

Mrs. Renee

William Marshall

The King of Cartoons

Phil Hartman

Captain Carl

Gilbert Lewis

The King of Cartoons

Shirley Stoler

Mrs. Steve

Gregory Harrison

Conky 2000 (voice) / Knucklehead (voice)

Natasha Lyonne

Opal

Diane Yang

Cher

Alisan Porter

Li'l Punkin

Johann Carlo

Dixie

Shaun Weiss

Elvis

Calvert DeForest

Roland Rodriquez

Tito

Leslie Jordan

Bubsy